A major difficulty associated with the assay of water samples for Cryptosporidium and Giardia is the presence of interfering particulate matter. Treatments are available for some of these materials but clay and other minerals remain a problem. This problem became acute during work on a pilot-scale, slow sand filtration plant which was being tested for its ability to remove Cryptosporidium oocysts from water. The plant was supplied with water seeded with oocysts and samples were taken both before and after treatment to determine the numbers present. Unfortunately, the raw water contained a small quantity of colloidal clay which led to the failure of the assay method employed. This paper presents the results of investigations undertaken to find a more suitable means of assay. Fluorescence-activated cell sorting, immunomagnetic separation and filter/laserscanning were used to identify oocysts.
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Published: 01/01/1997 ISBN(s): 0898679583 Number of Pages: 5File Size: 1 file , 100 KB